Dynamics of Bt cotton Cry1Ac protein content under an alternating high temperature regime and effects on nitrogen metabolism

This study was conducted to investigate the effects of alternating high temperature on Cry1Ac protein content on Bt cotton cultivars Sikang 1(SK-1,a conventional cultivar)and Sikang 3(SK-3,a hybrid cultivar).In 2011 and 2012,cotton plants were subjected to high temperature treatments ranging from 32 to 40℃ in climate chambers to investigate the effects of high temperature on boll shell insecticidal protein expression.The experiments showed that significant decline of the boll shell insecticidal protein was detected at temperatures higher than 38℃ after 24 h.Based on the results,the cotton plants were treated with the threshold temperature of 38℃ from 6:00 a.m.to 6:00 p.m.followed by a normal temperature of 27℃ during the remaining night hours(DH/NN)in 2012 and 2013.These treatments were conducted at peak boll growth stage for both cultivars in study periods of 0,4,7,and 10 d.Temperature treatment of 32℃ from 6:00 a.m.to 6:00 p.m.and 27℃ in the remaining hours was set as control.The results showed that,compared with the control,after the DH/NN stress treatment applied for 7 d,the boll shell Cry1Ac protein content level was significantly decreased by 19.1 and 17.5% for SK-1 and by 15.3 and 13.7% for SK-3 in 2012 and 2013,respectively.Further analysis of nitrogen metabolic physiology under DH/NN showed that the soluble protein content and the glutamic pyruvic transaminase(GPT)activities decreased slightly after 4 d,and then decreased sharply after 7 d.The free amino acid content and the protease content increased sharply after 7 d.The changes in SK-1 were greater than those in SK-3.These results suggest that under DH/NN stress,boll shell Cry1Ac protein content decline was delayed.Reduced protein synthesis and increased protein degradation in the boll shell decreased protein content,including Bt protein,which may reduce resistance to the cotton bollworm.     

开垦年限对黑土氮初级转化速率和净转化速率的影响

以东北黑土区开垦2 a和开垦30 a的典型旱作土壤为研究对象,采用15N同位素成对标记技术开展室内培养试验,利用数值计算模型(FLUAZ)计算不同开垦年限土壤的氮初级转化速率,以比较不同开垦年限黑土氮初级转化速率和净转化速率的差异,明确开垦年限对黑土氮转化过程的影响。结果表明,与开垦2a土壤相比,开垦30a土壤的有机碳和水溶性有机碳含量显著降低,导致土壤氮初级矿化速率和初级固定速率也显著降低。但开垦30a土壤的初级硝化速率、净硝化速率和净氮矿化速率却显著高于开垦2a土壤。两个开垦年限土壤的初级硝化速率分别为净硝化速率的1.15倍和1.02倍,说明土壤微生物对硝态氮的固定很少。开垦30a土壤的m/i值(氮初级矿化速率与初级固定速率之比)和n/ia值(初级硝化速率与初级铵态氮固定速率之比)均显著大于1,而开垦2 a土壤的m/i值和n/ia值均接近1。表明开垦2 a土壤的氮矿化与固定过程紧密偶联,氮素损失的风险较小,而开垦30 a土壤中氮矿化量超过了固定量,这为硝化作用的进行提供了底物,增加了硝酸盐反硝化和淋溶风险。     

森林土壤动物生态功能研究进展

森林土壤动物是森林土壤生态系统中不可分割的重要组成部分，对森林土壤生态系统的物质循环和能量流动起着重要作用。文中综述了近10年森林土壤动物生态功能的相关研究进展，包括森林土壤动物对土壤理化性质、微生物、植物的影响以及外来土壤动物入侵对土壤生态系统的影响等；展望了我国森林土壤动物的未来研究方向，未来研究应多关注森林土壤动物生态功能的作用机制、森林土壤动物对全球变化的响应、外来土壤动物入侵对森林生态系统的影响、森林土壤动物种类的研究范围以及新技术的应用，以期为我国森林土壤动物生态功能、土壤动物与生态系统、可持续利用土壤动物等领域研究提供科学参考。     

How do hedgerows influence soil organic carbon stock in livestock‐grazed pasture?

Hedgerows have the potential to influence ecosystem function in livestock‐grazed pasture. Despite this, they are often ignored when quantifying farmland ecosystem service delivery. In this study, we assess the contribution of hedgerows to the ecosystem function of carbon (C) storage, with a particular emphasis on soil organic carbon (SOC). We measured SOC stock (kg C m^?2), on an equivalent soil mass basis, at 0–0.15 m depth in pasture adjacent to 38 hedgerows (biotic) and 16 stone walls or fences (abiotic controls) across ten farms in the county of Conwy, Wales, UK. Pasture SOC stock (~7 kg C m^?2) was similar adjacent to biotic and abiotic field boundaries, positively associated with soil moisture and negatively with soil bulk density (BD). For biotic boundaries, two further variables were significantly associated with SOC stock, distance from hedgerow (decrease in SOC stock) and slope orientation (upslope SOC stock greater than downslope). For pasture adjacent to hedgerows, a model combining the aforementioned variables (BD, soil moisture, distance from hedgerow, slope orientation) explained 78% of variation in SOC stock. This study demonstrates that whilst hedgerows do have subtle positive effects on SOC stock in adjacent pasture, SOC storage adjacent to field boundaries is influenced more by soil moisture content and BD than field boundary type.     

我国主要麦区农户施肥评价及减肥潜力分析

【目的】明确我国主要麦区农户小麦施肥存在的问题及减肥潜力,为科学施肥、合理减肥提供依据。【方法】连续3年对我国主要麦区的小麦种植户进行施肥调研和取样,基于农户产量、养分需求量和土壤养分供应水平对其施肥状况和减肥潜力进行评价和分析。【结果】我国主要麦区农户小麦产量和生物量平均为6.0和13.2 t·hm ^-2,二者极显著线性相关。小麦产量与施肥量和土壤养分无显著相关。我国小麦氮（N）、磷（P₂O₅）和钾（K₂O）肥用量平均分别为191.1、112.8和53.4 kg·hm ^-2,春麦区农户氮、磷和钾肥用量平均分别为171.7、108.9和10.6 kg·hm ^-2,旱作区分别为154.3、111.8和32.6 kg·hm ^-2,麦玉区分别为236.4、128.1和74.0 kg·hm ^-2,稻麦区分别为177.5、77.0和71.8 kg·hm ^-2。就施氮量而言,春麦区过量施氮的农户较少,为34%,其次是麦玉区、稻麦区和旱作区,分别为42%、55%和63%;产量较低的农户是氮肥减施的重点,减氮潜力最高达43.6%,平均需减氮2.3—135.5 kg·hm ^-2。过量施磷问题比较突出,各麦区施磷过量的农户分别占63%、87%、68%和57%,即使小麦高产时,仍有超过50%的农户施磷过量;各麦区不同产量等级的农户均需减施磷肥,减磷量平均为3.8—91.1 kg·hm ^-2,旱作区减磷潜力最大,达55.6%。施钾状况因麦区而异,在春麦区,主要问题是施钾不足,占84%,平均需增施钾肥22.8 kg·hm ^-2;旱作、麦玉和稻麦区,减钾潜力分别达43.2%、25.7%和56.0%;产量较低的农户是减钾的重点,平均需减钾31.7—45.9 kg·hm ^-2。【结论】我国农户施肥状况和减肥潜力因农户产量和麦区不同存在差异,中低产农户过量施肥问题较为严重,应注意根据产量适量减少施用氮、钾肥,所有农户均需警惕磷肥过量投入问题,其中旱作区氮、磷肥减施潜力最高,稻麦区减钾潜力最高。     

水分优化对温室葡萄产量及土壤生物学特性的影响

为揭示滴灌不同水分调控下设施栽培葡萄产量与土壤学特性等相关指标的响应关系,在甘肃省永登县设施葡萄试验基地开展葡萄滴灌不同生育期水分调控田间灌溉试验,分析水分调控对设施栽培葡萄土壤积温、蔗糖酶和淀粉酶活性、有机碳(TOC)、微生物量碳(MBC)和微生物熵(qMB)等土壤学特性的影响,基于隶属函数综合分析明确影响葡萄产量及各项土壤生物学特性指标的最佳水分优化处理。结果表明:新梢期水分胁迫的土壤积温、土壤酶、TOC、MBC、qMB均显著(P <0. 05)高于对照;新梢期水分胁迫较对照可增产44. 6%;果实膨大期作为葡萄生长的关键阶段,此时水分胁迫对土壤淀粉酶影响不显著,但对其他土壤学特性指标具有一定的抑制作用,并且可减产9.7%;隶属函数综合分析表明,土壤生物学特性指标及产量综合隶属排名为:PS﹥FS﹥CS﹥CK﹥ES。     

氮磷减量与液体有机肥配施对膜下滴灌棉田土壤养分的影响

以新疆膜下滴灌棉田为试材,研究氮磷减量与液体有机肥配施对膜下滴灌棉田土壤养分的影响。试验设11个处理:(1)常规施肥(NP);(2)80%常规施肥(80%NP);(3)80%常规施肥+低量氨基酸有机肥(80%NP+LAA);(4)80%常规施肥+中量氨基酸有机肥(80%NP+MAA);(5)80%常规施肥+高量氨基酸有机肥(80%NP+HAA);(6)80%常规施肥+低量黄腐酸有机肥(80%NP+LFA);(7)80%常规施肥+中量黄腐酸有机肥(80%NP+MFA);(8)80%常规施肥+高量黄腐酸有机肥(80%NP+HFA);(9)80%常规施肥+低量沼液有机肥(80%NP+LBS);(10)80%常规施肥+中量沼液有机肥(80%NP+MBS);(11)80%常规施肥+高量沼液有机肥(80%NP+HBS);分别在蕾期、花期、成熟期采集0～20、20～40 cm土层土壤,测定土壤pH值、有机质、氮、磷、钾等有效养分含量。研究结果表明:(1)蕾期时,80%常规施肥配施高量黄腐酸、沼液有机肥显著降低了0～20 cm土层土壤pH值,并对提高土壤速效氮、速效磷含量效果最好。(2)花期时,80%常规施肥配施高量沼液有机肥对提高0～20 cm土层土壤有机质、速效磷含量效果最好。(3)成熟期时,80%常规施肥配施高量沼液有机肥对降低0～20 cm土层土壤pH值、提高土壤有机质含量效果最好。综上所述,新疆滴灌棉田应用80%常规施肥配施高量沼液有机肥效果最好,是新疆滴灌棉田提高土壤肥力和肥料利用率的有效途径。     

基于季节特征的土壤退墒模型建立与率定

利用河南省南阳市2011—2015年12个墒情站的土壤水分监测资料,分析研究了无增墒情况下土壤含水量消退规律,构建出基于不同季节(夏季、春秋季与冬季)的土壤含水量与衰减系数的数学模型,运用规划求解法率定模型参数,并利用2016—2017年资料对模型进行检验。结果表明,不同季节土壤水分衰竭系数模型分别为α=1.023(1-ω~2/4353)~(1/2)(夏季)、α=1.013(1-ω~2/7005)~(1/2)(春秋季)与α=1.008(1-ω~2/9303)~(1/2)(冬季),所建立的数学模型适用于南阳市相应季节壤土与粘壤土小麦、水稻与休闲地土壤旱情预测,但不适用于夏季与春秋季砂土特别是休闲地砂土土壤墒情预测。